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Assay Method Information |
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| | DNA-Dependent ATPase Assay |
| Description: | The hydrolysis of ATP catalyzed by PfUDN was assayed by measuring the formation of Pi from [γ-32P] ATP. The reaction mixture of 10 μl contained [γ-32P] ATP (specific activity 222 TBq. mmol^−1) and cold ATP (1 mM), ATPase buffer (20 mM Tris-HCl, pH 8.0, 8 mM DTT, 1.0 mM MgCl2, 20 mM KCl and 16 μg/ml BSA), purified PfUDN and 50 ng or 100 ng of M13 mp19 ssDNA. The reaction mixtures were incubated at 37°C for 60 min. |
| Affinity data for this assay | |
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