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| | Enzyme Inhibition Assay |
| Description: | The APAO and SMO activities were measured spectrophotometrically by following the formation of a pink adduct (ε515 nm = 2.6 × 10^4 M−1 cm−1) as the result of theH2O2-dependent oxidation of 4-aminoantipyrine catalyzed by horseradish peroxidase, and the subsequent condensation of oxidized 4-aminoantipyrine with 3,5-dichloro-2-hydroxybenzenesulfonic acid, as described in Bianchi et al. APAO activity was assayed at pH 7.5 (0.1 M Tris-HCl buffer) and 25°C with N1-acetyl-Spm as the substrate in the presence and absence of CHX. SMO activity was assayed at pH 8.3 (0.1 M Tris-HCl buffer) and 25°C using Spm as the substrate in the presence and absence of CHX. |
| Affinity data for this assay | |
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