Search and Browse
Download
Enter Data
|
Assay Method Information |
|
| | AChE Inhibition Assay |
| Description: | Inhibitory activities of synthesized α-oxycarbanilinophosphonates were determined at 25°C by the colorimetric method of Ellman et al. [Ellman et al., Biochem. Pharmacol., 7:88-95]. The assay solution contained 0.1 M phosphate buffer, pH 8, 0.3 mM 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB), 0.125 units of AChE (Sigma Chemical Co. from electric eel (Electrophorous electricus)), and 0.2 mM acetylthiocholine iodide as the substrate in a total volume of 2.5 mL. In order to provide sufficient time for interaction with either PAS and/or active site of the enzyme, particularly in competition with the substrate, the synthesized compounds were added to the assay solution and preincubated with the enzyme for 10 min at 25°C. In order to solubilize the synthesized compounds, the reaction mixture contained 2% ethanol (50 μL in 2.5 mL reaction mixture volume). Control experiments were run and it was seen that no inhibitory effect was exerted on the enzyme by ethanol. The changes in abs |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |
|
Home |
| |
Search |
| |
Deposit |
| |
SiteMap |
| |
About us |
| |
Email us |
| |
Info |
|
©2000 BindingDB. All rights reserved. |
|||||||||||||