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| | Esterase Activity Assay |
| Description: | CA activity was assayed by following the change in absorbance at 348 nm of 4-nitrophenylacetate (NPA) to 4-nitrophenylate ion over a period of 3 min at 25 °C using a spectrophotometer (Shimadzu, UVmini-1240 UV-VIS spectrophotometer) according to the method described by Verpoorte et al.37 The enzymatic reaction contained 1.4mL 0.05M Tris-SO4 buffer (pH 7.4), 1mL 3mM 4-NPA, 0.5mL H2O and 0.1mL enzyme solution (total volume, 3.0 mL). A reference measurement was obtained bypreparing the mixture without the enzyme solution. All measurements were recorded in triplicate. |
| Affinity data for this assay | |
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