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| | Patch Clamp/Inhibition Assay |
| Description: | Cells and methods: Functional inhibition of the sodium currents is studied using whole cell patch clamp methods (Hamill O. P., Marty A., Neher E., Sakmann B., Sigworth F. J., Pflugers Arch. 391 (2): 85-100 (1981)) on the hybrid cell line ND7/23 (Wood J N, Bevan S J, Coote P R, Dunn P M, Harmar A, Hogan P, Latchman D S, Morrison C, Rougon G, Theveniau M.: "Novel cell lines display properties of nociceptive sensory neurons". Proc. Biol. Sci. Sep 22; 241(1302):187-94 (1990)), that express a mixed population of voltage gated sodium channels. For sodium current recording control bath solution contained (mM): NaCl (80), choline chloride (38), CaCl2 (1.3), MgCl2 (2), KCl (2), CdCl2 (0.4), NiCl2 (0.3), TEA.Cl (20), Hepes (10), glucose (10). Internal pipette solution consists of (mM): CsF (65), CsCl (65), NaCl (10), CaCl2 (1.3), MgCl2 (2), Hepes (10), EGTA (10), MgATP (1). Compounds are dissolved as 20 mM stock solutions in DMSO and then diluted to the final concentration in the external solutions. |
| Affinity data for this assay | |
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