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Assay Method Information |
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| | Inhibition Assay |
| Description: | 1× Kinase Buffer 50 mM HEPES. pH 7.5 0.0015% Brij-35 10 mM MgCl2 2 mM DTT 2) stop buffer 100 mM HEPES, pH 7.5 0.015% Brij-35 0.2% Coating Reagent #3 50 mM EDTAPrepare a Compound Solution to be Tested Step 2.1 Dilute the compound to 50× of the final desired highest inhibitor concentration in reaction by 100% DMSO. Transfer 100 μl of this compound dilution to a well in a 96-well plate. For example, if desired highest inhibitor concentration is 1 μM, then prepare 50 μM of compound DMSO solution in this step. |
| Affinity data for this assay | |
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