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| | IC50 Value Determination |
| Description: | IC50 values were determined for compounds that covalently inhibit SARS-CoV-2 3CLpro using our recently described assay and data fitting methods that were derived from our previous study on SARS-CoV 3CLpro and inhibition by chloropyridyl esters. The only difference was that the preincubation time of the enzyme with the compounds was 10 min instead of 20 min. In addition, the Morrison equation was only used to determine the IC50 value when the concentration was below 1 μM. |
| Affinity data for this assay | |
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