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Assay Method Information |
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| | Biochemical Assay |
| Description: | IC50 values were determined using the Z′-LYTE activity assay, with ATP concentration used at Km app for the specific assay (500 μM ATP for CSF-1R (FMS) and 75 μM ATP for KDR (VEGFR2)).The Z′-LYTE biochemical assay employs a fluorescence-based, coupled-enzyme format and is based on the differential sensitivity of phosphorylated and non-phosphorylated peptides to proteolytic cleavage. The peptide substrate is labelled with two fluorophores one at each end that make up a FRET (fluorescence resonance energy transfer) pair. |
| Affinity data for this assay | |
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