Assay Method Information |
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| ChEMBL_2022242 (CHEMBL4676055) |
Description: | Inhibition of 1 nM recombinant human N-terminal His-tagged KHKC expressed in Escherichia coli BL21 (DE3) using fructose as substrate preincubated for 30 mins followed by ATP addition and measured for 3 hrs by pyruvate kinase-lactate dehydrogenase coupled assay |
Affinity data for this assay | |
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