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| | Kinase Inhibition Assay |
| Description: | In vitro kinase inhibition assay using purified GSK-3 alpha from insect cells, was incubated at room temperature with substrate, and test compounds in the presence of ATP/ [gamma-33P]. The total ATP concentration was 10 µM (IC50 determinations) or ranged from 0 to 45 µM (Ki determinations). 33P incorporation into the substrate peptide was determined by using a scintillation counter (Wallac). |
| Affinity data for this assay | |
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