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Assay Method Information |
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| | Biochemical Assay for Inhibitors of CLK2 Kinase Activity Assay |
| Description: | The assay was conducted in the same way as described for CK2α, with final assay conditions comprising 20 nM CLK2 (Carna Biosciences-04-127), 50 μM peptide substrate (KRRRLASLR), 100 μM ATP in 1× reaction buffer (40 mM Tris pH7.5, 200 mM NaCl, 20 mM MgCl2, 0.1 mg/mL BSA, 1% DMSO). |
| Affinity data for this assay | |
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