Assay Method Information |
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| D2R Binding Assay |
Description: | Human embryonic kidney (HEK-293) cells were plated in 100 mm plates and transfected with pcDNA3.1-D2R using Lipofectamine 2000 (Invitrogen; Waltham, MA) according to the manufacturer's instructions. Radioligand binding assays were performed 24 h after transfection essentially as described. |
Affinity data for this assay | |
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