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Assay Method Information |
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| | Biological Assay |
| Description: | Two types of enzyme assays were developed to follow TgCDPK1 activity, a radiometric scintillation proximity assay measured the labeled γ-phosphate of ATP added to a biotinylated peptide substrate and an ATP consumption assay where ATP consumption was monitored by luciferase and light production (KinaseGlo , Promega Corp., Madison, Wis.). Both assays gave similar results for calcium dependence, Km of substrates (less than 2-fold differences in Km values; see Table 6 FIGS. 6, 7, 8, and 9), and inhibitor concentrations for 50% enzyme inhibition. |
| Affinity data for this assay | |
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