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Assay Method Information

Assay Name:  Radioligand Binding Assay
Description:  A scintillation proximity assay was used for radioligand competition and saturation binding assays. Nonspecific binding was determined in the presence of 1 uM PGD2. Binding activity was determined by using a 1450 Microbeta scintillation counter (Wallac, UK). Ki values were calculated by using the Cheng-Prusoff equation, and represent the average of at least three independent dose-response experiments.
Affinity data for this assay
 

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Last update November 1, 2007
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