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| | PLK3 Kinase Inhibition Assay |
| Description: | The mode of human PLK3 inhibition by LFM-A13 was examined in titration experiments using increasing concentrations of [gamma-32P]ATP and purified N-terminal His6-tagged recombinant human PLK3. The Ki of PLK3 by LFM-A13 was calculated from the reciprocal plots of the intensity of phosphorylation of the substrate versus the concentration of the inhibitor. From this Dixon plot, the Ki represents the dissociation constants of the EI complex, which is determined by the point of linear intersection. |
| Affinity data for this assay | |
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