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| | Radioligand Binding Assay |
| Description: | Compounds were tested for their inhibitory effects on ligand binding to the human ORL1 receptor. Bound and free radioligands are separated by filtration using a unifilter plate GF/C-96. The remaining radioactivity on the filter was counted using a TopCount-HTS (PerkinElmer). Specific binding of [125I][Tyr14]nociceptin is defined as the difference between total binding and nonspecific binding in the presence of 1 uM nociceptin. J-113397 was used as internal control across all assay plates for internal validation. IC50 values are calculated using GraphPad Prism (Ver.3.03). |
| Affinity data for this assay | |
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