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| | SPA Binding Assay (IC50) and Coactivator Peptide Competitive FP Assay (IC50/EC50) |
| Description: | A radiolabeled ligand competition scintillation proximity assay (SPA) for the androgen receptor (AR) using Ni-coated 384-well FlashPlates. It measures [3H]-DHT displacement from AR-LBD protein. AR functionality was determined by fluorescence polarization (excitation @485 nm, emission @ 530 nm) assay, which measures fluorescently labeled SRC2-3 peptide displacement from DHT-bound AR-LBD. |
| Affinity data for this assay | |
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