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Assay Method Information |
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| | Luciferase-Based Assay |
| Description: | Pdgfrβ (0.16 ug/well, Panvera/Invitrogen) 500 nM ATP and the PTK2 peptide (700 uM) were combined with compound and reaction buffer as noted above for src. The reaction was incubated for 60 minutes at 37 C, and the residual ATP concentration was determined using the luciferase-based technique. |
| Affinity data for this assay | |
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