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| | MAO-A/B Activity and Inhibition Assay |
| Description: | Reactions (100 µL) were initiated by the addition of 2 µL MAO-A/B (final concentrations were 100-200 nM and 0.837 µM for MAO-A and MAO-B, respectively). The reaction mixtures contained 50 mM HEPES (pH 7.5), 0.1 mM 4-aminoantipyrine, 1 mM 3,5-dichloro-2-hydroxybenzenesulfonic acid, 0.04 mg/mL horseradish peroxidase, and appropriate concentration of buffered substrate (tyramine). Absorbance changes were measured at 515 nm. |
| Affinity data for this assay | |
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