Assay Method Information |
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| Spectral Binding Titrations |
Description: | Ligand binidng to CYP3A4 was monitored in 50 mM phosphate (pH 7.4) containing 20% glycerol and 1 mM DTT. The protein was titrated with small aliquots of DMSO solutions of GS4, GS5, GS6, GS7, or GS8 with the final solvent concentration < 2%. |
Affinity data for this assay | |
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