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Assay Method Information

Assay Name:  HDAC8 Inhibition Assay (kinetic method)
Description:  To determine the dissociation rate of the inhibitor, 1 µM HDAC8 and 20 µM inhibitor (syringe I) were mixed with 100 µM c-SAHA (syringe II) via the stopped-flow system. The fluorescence was measured at 325 nm with a 395 nm cutoff filter.
Affinity data for this assay
 

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Last update November 1, 2007
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