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Assay Method Information |
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| | P. aeruginosa MurC assay |
| Description: | The reactions (50 μL) were carried out in 50 mM Tris-HCl pH 8.0, 20 mM ammonium sulfate, 2.5 mM DTT, 0.002% Brij-35, 1 mM MgCl2, 18 μM UNAM, 38 μM ATP, 28 μM L-alanine, 2 μg/mL Poly uridine nucleic acid, 0.1 μM Polynucleotide Phosphorylase, 0.25X ribogreen and 3 nM enzyme in 384-well plate at 25 C for 60 minutes. |
| Affinity data for this assay | |
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