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Assay Method Information |
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| | ITC |
| Description: | SmARG was dialyzed against 50 mM bicine (pH 8.5), 100 μM MnCl2, and 1.0 mM TCEP [5% (v/v) DMSO was included in the dialysis buffer when SmARG was titrated with ABH-PE and ABH-DP]. The inhibitor (ABH, 0.547 mM; NOHA, 1.097 mM; nor-NOHA, 0.443 mM; ABH-PE, 0.410 mM; ABH-DP, 0.436 mM) was dissolved in dialysis buffer. |
| Affinity data for this assay | |
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