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Assay Method Information |
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| | Isothermal Titration Calorimetry (ITC) |
| Description: | ITC experiments were carried out at an enzyme [E] concentration of 25-50 μM with 0.25-1 mM compound [I] concentration in the KDM5 storage buffer with ~500 μM MnCl2 and 10% (v/v) DMSO. A MicroCal Auto-iTC200 was used to perform 25 4-μL injections of compounds into protein or buffer (to measure the heat of dilution) |
| Affinity data for this assay | |
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