Assay Method Information |
|
| Proteros Reporter Displacement Assay |
Description: | Specifically, the experiments were conducted in black 384-well polypropylene plates (Corning Glass) in a 15-ul reaction volume consisting of 11 nM HDAC1 or 50 nM HDAC2 in reaction buffer (25 mM Tris, pH 8.0, 1 mM DTT, 150 mM NaCl, 0.01% Tween 20) and reporter probes at concentrations equal to their binding affinity (Kd). Probe binding was monitored for 240 min (4 h) with measurements recorded every 8 min. The decay rate of probe displacement was used to quantify the association rate constant for test inhibitors. |
Affinity data for this assay | |
---|---|
If you find an error in this entry please send us an E-mail |
Home |
| |
Search |
| |
Deposit |
| |
SiteMap |
| |
About us |
| |
Email us |
| |
Info |
|
©2000 BindingDB. All rights reserved. |