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Assay Method Information

Assay Name:  Proteros Reporter Displacement Assay
Description:  Specifically, the experiments were conducted in black 384-well polypropylene plates (Corning Glass) in a 15-ul reaction volume consisting of 11 nM HDAC1 or 50 nM HDAC2 in reaction buffer (25 mM Tris, pH 8.0, 1 mM DTT, 150 mM NaCl, 0.01% Tween 20) and reporter probes at concentrations equal to their binding affinity (Kd). Probe binding was monitored for 240 min (4 h) with measurements recorded every 8 min. The decay rate of probe displacement was used to quantify the association rate constant for test inhibitors.
Affinity data for this assay
 

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Last update November 1, 2007
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