Assay Method Information |
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| VEGF-R Kinase Inhibition Assay |
Description: | Tha assay was using human VEGFR-2 in DELFIA (dissociation-enhanced lanthanide fluoroimmunoassay) format. IC50 is the concentration of inhibitor that inhibits 50% of kinase activity that catalyzes the transfer of the terminal phosphate to the the copolymer substrate. Compounds are tested using halflog dilutions from 10 through 0.0001 uM. IC50 values are calculated by least squares regression using the Hill equation for the best fit of all the points in the dose response curve. |
Affinity data for this assay | |
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