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Assay Method Information

Assay Name:  ChEMBL_1854944 (CHEMBL4355673)
Description:  Inhibition of recombinant full length C-terminal His/FLAG tagged human HDAC1 (1 to 482 residues) expressed in baculovirus infected sf9 insect cells using p53 (379 to 382 residues) derived fluorogenic peptide RHKKAc as substrate preincubated for 5 to 10 mins followed by substrate addition and further incubated for 2 hrs by fluorescence assay
Affinity data for this assay
 

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Last update November 1, 2007
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