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Assay Method Information |
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| | ChEMBL_1824605 (CHEMBL4324369) |
| Description: | Inhibition of full-length human N-terminal TEV cleavage site NNMT (1 to 270 residues) expressed in Escherichia coli BL21-CodonPlus(DE3)-RIPL cells using nicotinamide as substrate preincubated for 30 mins in presence of AdoMet followed by substrate addition by SAHH enzyme coupled fluorescence assay based Morrison equation analysis |
| Affinity data for this assay | |
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