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Assay Method Information |
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| | SPR Directy Binding Assay (Biacore 4000) |
| Description: | The protocols for tight and weak binders were described in Biacore S51 assay set-up with a flow rate of 30 µL/min. For His-Bcl-2 protein assays, the running buffer contained 0.01 M HEPES, 0.15 M NaCl, 50 µM EDTA, 0.05% P20 and 1% DMSO at pH 7.4. The His-Bcl-2 protein surface was regenerated between binding cycles with at least a 5-min injection of running buffer for tight-binding compounds. |
| Affinity data for this assay | |
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