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Assay Method Information |
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| | ChEMBL_1873873 (CHEMBL4375162) |
| Description: | Inhibition of N-terminal His6-tagged human PHGDH expressed in Escherichia coli Rosetta (DE3)pLysS assessed as effect on NADH fluorescence incubated for 20 mins using 3-PG as substrate in presence of NAD+ and PSAT1 by diaphorase and resazurin dye based fluorescence assay |
| Affinity data for this assay | |
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