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Assay Method Information |
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| | ChEMBL_951484 (CHEMBL2350637) |
| Description: | Competitive inhibition of human recombinant DOT1L (1 to 420 amino acid residues) overexpressed in Escherichia coli BL21 (DE3) using [3H]-SAM as substrate assessed as inhibition of nucleosome methylation incubated for 30 mins prior to substrate addition measured after 1 hr by scintillation counting analysis |
| Affinity data for this assay | |
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