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Assay Method Information

Assay Name:  ChEMBL_951484
Description:  Competitive inhibition of human recombinant DOT1L (1 to 420 amino acid residues) overexpressed in Escherichia coli BL21 (DE3) using [3H]-SAM as substrate assessed as inhibition of nucleosome methylation incubated for 30 mins prior to substrate addition measured after 1 hr by scintillation counting analysis
Affinity data for this assay
 

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Last update November 1, 2007
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