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| | Cell-Based Functional Assay (M1) |
| Description: | Muscarinic acetylcholine receptor M1 was cloned into an expression vector and transfected in CHO cells along with CRE-Luc reporter system. Isolated colonies were screened with 10,000 nM acetylcholine. Clones exhibiting highest acetylcholine dependent reporter gene induction were utilized in the evaluation of known and novel compounds. Increasing concentrations of the compound were incubated with or without 10,000 nM acetylcholine for antagonist or agonist mode, respectively, for 5 h in 96 well plate. After incubation, cells were harvested, lysed and reporter gene activity was determined. |
| Affinity data for this assay | |
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