Found 32 with Last Name = 'idamakanti' and Initial = 'n' TargetSerine/threonine-protein kinase WNK1(Homo sapiens (Human))
Novartis Institutes for BioMedical Research, Inc. , Cambridge, Massachusetts 02139-4133, United States.
Curated by ChEMBL
Novartis Institutes for BioMedical Research, Inc. , Cambridge, Massachusetts 02139-4133, United States.
Curated by ChEMBL
Affinity DataKi: 32nMAssay Description:Non-competitive inhibition of recombinant human N-terminal GST-tagged WNK1 (1 to 491 residues) expressed in baculovirus expression system using fluor...More data for this Ligand-Target Pair
Affinity DataIC50: 1nMpH: 7.3 T: 2°CAssay Description:Each well of the MBP-coated ScintiPlates held 100 ul of a solution containing 20 mM HEPES pH 7.3, 5 mM MnCl2 (WNK1 and WNK4) or 3 mM MnCl2 (WNK2 and ...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase WNK1(Homo sapiens (Human))
Novartis Institutes for BioMedical Research, Inc. , Cambridge, Massachusetts 02139-4133, United States.
Curated by ChEMBL
Novartis Institutes for BioMedical Research, Inc. , Cambridge, Massachusetts 02139-4133, United States.
Curated by ChEMBL
Affinity DataIC50: 4nMAssay Description:Allosteric inhibition of recombinant human N-terminal GST-tagged WNK1 catalytic domain (1 to 491 residues) expressed in baculovirus expression system...More data for this Ligand-Target Pair
Affinity DataIC50: 5nMpH: 7.3 T: 2°CAssay Description:Each well of the MBP-coated ScintiPlates held 100 ul of a solution containing 20 mM HEPES pH 7.3, 5 mM MnCl2 (WNK1 and WNK4) or 3 mM MnCl2 (WNK2 and ...More data for this Ligand-Target Pair
Affinity DataIC50: 6nMpH: 7.3 T: 2°CAssay Description:Each well of the MBP-coated ScintiPlates held 100 ul of a solution containing 20 mM HEPES pH 7.3, 5 mM MnCl2 (WNK1 and WNK4) or 3 mM MnCl2 (WNK2 and ...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase WNK1(Homo sapiens (Human))
Novartis Institutes for BioMedical Research, Inc. , Cambridge, Massachusetts 02139-4133, United States.
Curated by ChEMBL
Novartis Institutes for BioMedical Research, Inc. , Cambridge, Massachusetts 02139-4133, United States.
Curated by ChEMBL
Affinity DataIC50: 6nMAssay Description:Allosteric inhibition of WNK1 (unknown origin) expressed in HEK293 cells co-expressing flag-OSR1 assessed as reduction in sorbitol-stimulated OSR1 ph...More data for this Ligand-Target Pair
Affinity DataIC50: 9nMpH: 7.3 T: 2°CAssay Description:Each well of the MBP-coated ScintiPlates held 100 ul of a solution containing 20 mM HEPES pH 7.3, 5 mM MnCl2 (WNK1 and WNK4) or 3 mM MnCl2 (WNK2 and ...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase WNK1(Homo sapiens (Human))
Novartis Institutes for BioMedical Research, Inc. , Cambridge, Massachusetts 02139-4133, United States.
Curated by ChEMBL
Novartis Institutes for BioMedical Research, Inc. , Cambridge, Massachusetts 02139-4133, United States.
Curated by ChEMBL
Affinity DataIC50: 39nMAssay Description:Allosteric inhibition of WNK1 (unknown origin) expressed in HEK293 cells co-expressing flag-OSR1 assessed as reduction in sorbitol-stimulated OSR1 ph...More data for this Ligand-Target Pair
3D Structure (crystal)Affinity DataIC50: 41nMpH: 7.3 T: 2°CAssay Description:Each well of the MBP-coated ScintiPlates held 100 ul of a solution containing 20 mM HEPES pH 7.3, 5 mM MnCl2 (WNK1 and WNK4) or 3 mM MnCl2 (WNK2 and ...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase WNK2 [166-489](Homo sapiens (Human))
Novartis Institutes for BioMedical Research, Inc.
Novartis Institutes for BioMedical Research, Inc.
Affinity DataIC50: 50nMpH: 7.3Assay Description:The assay utilized 5 to 10 nM of WNK1−4 protein compared to 25 nM used for mobility shift assay, enabling a more accurate comparison of selecti...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase WNK1(Homo sapiens (Human))
Novartis Institutes for BioMedical Research, Inc. , Cambridge, Massachusetts 02139-4133, United States.
Curated by ChEMBL
Novartis Institutes for BioMedical Research, Inc. , Cambridge, Massachusetts 02139-4133, United States.
Curated by ChEMBL
Affinity DataIC50: 60nMAssay Description:Allosteric inhibition of WNK1 (unknown origin) expressed in HEK293 cells co-expressing flag-OSR1 assessed as reduction in sorbitol-stimulated OSR1 ph...More data for this Ligand-Target Pair
Affinity DataIC50: 64nMpH: 7.3Assay Description:The assay utilized 5 to 10 nM of WNK1−4 protein compared to 25 nM used for mobility shift assay, enabling a more accurate comparison of selecti...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase WNK1(Homo sapiens (Human))
Novartis Institutes for BioMedical Research, Inc. , Cambridge, Massachusetts 02139-4133, United States.
Curated by ChEMBL
Novartis Institutes for BioMedical Research, Inc. , Cambridge, Massachusetts 02139-4133, United States.
Curated by ChEMBL
Affinity DataIC50: 95nMAssay Description:Allosteric inhibition of WNK1 (unknown origin) expressed in HEK293 cells co-expressing flag-OSR1 assessed as reduction in sorbitol-stimulated OSR1 ph...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase WNK1(Homo sapiens (Human))
Novartis Institutes for BioMedical Research, Inc. , Cambridge, Massachusetts 02139-4133, United States.
Curated by ChEMBL
Novartis Institutes for BioMedical Research, Inc. , Cambridge, Massachusetts 02139-4133, United States.
Curated by ChEMBL
Affinity DataIC50: 106nMAssay Description:Allosteric inhibition of WNK1 (unknown origin) expressed in HEK293 cells co-expressing flag-OSR1 assessed as reduction in sorbitol-stimulated OSR1 ph...More data for this Ligand-Target Pair
Affinity DataIC50: 150nMpH: 7.5Assay Description:A mixture of fluorescein labeled OSR1 peptide substrate (Toray Research Center, Inc.) and ATP was prepared with final concentrations of 10 and 25 ...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase WNK1(Homo sapiens (Human))
Novartis Institutes for BioMedical Research, Inc. , Cambridge, Massachusetts 02139-4133, United States.
Curated by ChEMBL
Novartis Institutes for BioMedical Research, Inc. , Cambridge, Massachusetts 02139-4133, United States.
Curated by ChEMBL
Affinity DataIC50: 162nMAssay Description:Allosteric inhibition of WNK1 (unknown origin) expressed in HEK293 cells co-expressing flag-OSR1 assessed as reduction in sorbitol-stimulated OSR1 ph...More data for this Ligand-Target Pair
Affinity DataIC50: 180nMpH: 7.5Assay Description:A mixture of fluorescein labeled OSR1 peptide substrate (Toray Research Center, Inc.) and ATP was prepared with final concentrations of 10 and 25 ...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase WNK4 [1-444](Homo sapiens (Human))
Novartis Institutes for BioMedical Research, Inc.
Novartis Institutes for BioMedical Research, Inc.
Affinity DataIC50: 187nMpH: 7.3Assay Description:The assay utilized 5 to 10 nM of WNK1−4 protein compared to 25 nM used for mobility shift assay, enabling a more accurate comparison of selecti...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase WNK3 [1-434](Homo sapiens (Human))
Novartis Institutes for BioMedical Research, Inc.
Novartis Institutes for BioMedical Research, Inc.
Affinity DataIC50: 377nMpH: 7.3Assay Description:The assay utilized 5 to 10 nM of WNK1−4 protein compared to 25 nM used for mobility shift assay, enabling a more accurate comparison of selecti...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase WNK1(Homo sapiens (Human))
Novartis Institutes for BioMedical Research, Inc. , Cambridge, Massachusetts 02139-4133, United States.
Curated by ChEMBL
Novartis Institutes for BioMedical Research, Inc. , Cambridge, Massachusetts 02139-4133, United States.
Curated by ChEMBL
Affinity DataIC50: 420nMAssay Description:Allosteric inhibition of WNK1 (unknown origin) expressed in HEK293 cells co-expressing flag-OSR1 assessed as reduction in sorbitol-stimulated OSR1 ph...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase WNK1(Homo sapiens (Human))
Novartis Institutes for BioMedical Research, Inc. , Cambridge, Massachusetts 02139-4133, United States.
Curated by ChEMBL
Novartis Institutes for BioMedical Research, Inc. , Cambridge, Massachusetts 02139-4133, United States.
Curated by ChEMBL
Affinity DataIC50: 623nMAssay Description:Allosteric inhibition of WNK1 (unknown origin) expressed in HEK293 cells co-expressing flag-OSR1 assessed as reduction in sorbitol-stimulated OSR1 ph...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase WNK1(Homo sapiens (Human))
Novartis Institutes for BioMedical Research, Inc. , Cambridge, Massachusetts 02139-4133, United States.
Curated by ChEMBL
Novartis Institutes for BioMedical Research, Inc. , Cambridge, Massachusetts 02139-4133, United States.
Curated by ChEMBL
Affinity DataIC50: 750nMAssay Description:Allosteric inhibition of WNK1 (unknown origin) expressed in HEK293 cells co-expressing flag-OSR1 assessed as reduction in sorbitol-stimulated OSR1 ph...More data for this Ligand-Target Pair
Affinity DataIC50: 856nMpH: 7.3 T: 2°CAssay Description:Each well of the MBP-coated ScintiPlates held 100 ul of a solution containing 20 mM HEPES pH 7.3, 5 mM MnCl2 (WNK1 and WNK4) or 3 mM MnCl2 (WNK2 and ...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase WNK1(Homo sapiens (Human))
Novartis Institutes for BioMedical Research, Inc. , Cambridge, Massachusetts 02139-4133, United States.
Curated by ChEMBL
Novartis Institutes for BioMedical Research, Inc. , Cambridge, Massachusetts 02139-4133, United States.
Curated by ChEMBL
Affinity DataIC50: 971nMAssay Description:Allosteric inhibition of WNK1 (unknown origin) expressed in HEK293 cells co-expressing flag-OSR1 assessed as reduction in sorbitol-stimulated OSR1 ph...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase WNK1(Homo sapiens (Human))
Novartis Institutes for BioMedical Research, Inc. , Cambridge, Massachusetts 02139-4133, United States.
Curated by ChEMBL
Novartis Institutes for BioMedical Research, Inc. , Cambridge, Massachusetts 02139-4133, United States.
Curated by ChEMBL
Affinity DataIC50: 2.14E+3nMAssay Description:Allosteric inhibition of WNK1 (unknown origin) expressed in HEK293 cells co-expressing flag-OSR1 assessed as reduction in sorbitol-stimulated OSR1 ph...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase WNK1 [198-401](Homo sapiens (Human))
Novartis Institutes for BioMedical Research, Inc.
Novartis Institutes for BioMedical Research, Inc.
Affinity DataIC50: 5.70E+3nMAssay Description:For hit validation, eight compound replicates were made from the original stock dilution plates. The Cybi-WellTM dispenser was used to prepare 1.5 &#...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase WNK1 [198-401](Homo sapiens (Human))
Novartis Institutes for BioMedical Research, Inc.
Novartis Institutes for BioMedical Research, Inc.
Affinity DataIC50: 8.80E+3nMAssay Description:For hit validation, eight compound replicates were made from the original stock dilution plates. The Cybi-WellTM dispenser was used to prepare 1.5 &#...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase WNK1 [198-401](Homo sapiens (Human))
Novartis Institutes for BioMedical Research, Inc.
Novartis Institutes for BioMedical Research, Inc.
Affinity DataIC50: 1.60E+4nMAssay Description:For hit validation, eight compound replicates were made from the original stock dilution plates. The Cybi-WellTM dispenser was used to prepare 1.5 &#...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase WNK1 [198-401](Homo sapiens (Human))
Novartis Institutes for BioMedical Research, Inc.
Novartis Institutes for BioMedical Research, Inc.
Affinity DataIC50: 8.00E+4nMAssay Description:For hit validation, eight compound replicates were made from the original stock dilution plates. The Cybi-WellTM dispenser was used to prepare 1.5 &#...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase WNK1(Homo sapiens (Human))
Novartis Institutes for BioMedical Research, Inc. , Cambridge, Massachusetts 02139-4133, United States.
Curated by ChEMBL
Novartis Institutes for BioMedical Research, Inc. , Cambridge, Massachusetts 02139-4133, United States.
Curated by ChEMBL
Affinity DataEC50: 589nMAssay Description:Allosteric inhibition of WNK1 (unknown origin) expressed in HEK293 cells co-expressing flag-OSR1 assessed as reduction in sorbitol-stimulated OSR1 ph...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase WNK1(Homo sapiens (Human))
Novartis Institutes for BioMedical Research, Inc. , Cambridge, Massachusetts 02139-4133, United States.
Curated by ChEMBL
Novartis Institutes for BioMedical Research, Inc. , Cambridge, Massachusetts 02139-4133, United States.
Curated by ChEMBL
Affinity DataEC50: 1.39E+3nMAssay Description:Allosteric inhibition of WNK1 (unknown origin) expressed in HEK293 cells co-expressing flag-OSR1 assessed as reduction in sorbitol-stimulated OSR1 ph...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase WNK1(Homo sapiens (Human))
Novartis Institutes for BioMedical Research, Inc. , Cambridge, Massachusetts 02139-4133, United States.
Curated by ChEMBL
Novartis Institutes for BioMedical Research, Inc. , Cambridge, Massachusetts 02139-4133, United States.
Curated by ChEMBL
Affinity DataEC50: 352nMAssay Description:Allosteric inhibition of WNK1 (unknown origin) expressed in HEK293 cells co-expressing flag-OSR1 assessed as reduction in sorbitol-stimulated OSR1 ph...More data for this Ligand-Target Pair
