Affinity DataKi: 7nM ΔG°: -11.6kcal/mole IC50: 1.24nMpH: 5.2 T: 2°CAssay Description:Enzymatic activity assay using fluorescent activity-based labeling method.More data for this Ligand-Target Pair
Affinity DataKi: 8nM ΔG°: -11.5kcal/mole IC50: 1.94nMpH: 5.2 T: 2°CAssay Description:Enzymatic activity assay using fluorescent activity-based labeling method.More data for this Ligand-Target Pair
Affinity DataIC50: 1.95nMAssay Description:The enzyme in GCase enzyme activity buffer (25 μL/well) was added to a 384-well black plate. The fluorescent probe 3 (25 nL/well, 50 nM final co...More data for this Ligand-Target Pair
Affinity DataKi: 0.950nM IC50: 2nMpH: 5.2Assay Description:Activity of GBA was measured at 37° C. with 4-methylumbelliferyl β-D-glucopyranoside as substrate as reported previously. To determine the IC50 ...More data for this Ligand-Target Pair
Affinity DataIC50: 2.04nMpH: 7.0Assay Description:The assays were performed at 37°C with 4-MU-β-ᴅ-Glu as the substrate in Mcllvaine buffer (sodium citrate (100mM) and sodium phosphate...More data for this Ligand-Target Pair
Affinity DataIC50: 2.40nMAssay Description:Various concentrations of test compounds were prepared in DMSO and then diluted into buffer consisting of 50 mM sodium phosphate 0.25% w/v sodium tau...More data for this Ligand-Target Pair
Affinity DataIC50: 2.40nMpH: 7.0Assay Description:Various concentrations of test compounds were prepared in DMSO and then diluted into buffer consisting of 50 mM sodium phosphate 0.25% w/v sodium tau...More data for this Ligand-Target Pair
Affinity DataIC50: 2.5nMpH: 7.0Assay Description:Various concentrations of test compounds were prepared in DMSO and then diluted into buffer consisting of 50 mM sodium phosphate 0.25% w/v sodium tau...More data for this Ligand-Target Pair
Affinity DataIC50: 2.5nMAssay Description:Various concentrations of test compounds were prepared in DMSO and then diluted into buffer consisting of 50 mM sodium phosphate 0.25% w/v sodium tau...More data for this Ligand-Target Pair
Affinity DataIC50: 2.97nMpH: 5.2Assay Description:The assays were performed at 37°C with 4-MU-β-ᴅ-Glu as the substrate in Mcllvaine buffer (sodium citrate (100mM) and sodium phosphate...More data for this Ligand-Target Pair
Affinity DataIC50: 3.62nMpH: 7.0Assay Description:The assays were performed at 37°C with 4-MU-β-ᴅ-Glu as the substrate in Mcllvaine buffer (sodium citrate (100mM) and sodium phosphate...More data for this Ligand-Target Pair
Affinity DataIC50: 3.70nMAssay Description:Various concentrations of test compounds were prepared in DMSO and then diluted into buffer consisting of 50 mM sodium phosphate 0.25% w/v sodium tau...More data for this Ligand-Target Pair
In DepthDetails
Affinity DataIC50: 3.70nMpH: 7.0Assay Description:Various concentrations of test compounds were prepared in DMSO and then diluted into buffer consisting of 50 mM sodium phosphate 0.25% w/v sodium tau...More data for this Ligand-Target Pair
Affinity DataIC50: 3.71nMpH: 5.2Assay Description:The assays were performed at 37°C with 4-MU-β-ᴅ-Glu as the substrate in Mcllvaine buffer (sodium citrate (100mM) and sodium phosphate...More data for this Ligand-Target Pair
Affinity DataIC50: 4nMpH: 5.2Assay Description:Competitive inhibition of recombinant Imiglucerase using 4-methylumbelliferyl-beta-D-glucopyranoside as substrate preincubated for 30 mins before sub...More data for this Ligand-Target Pair
Affinity DataIC50: 4nMpH: 5.2Assay Description:Competitive inhibition of recombinant Imiglucerase using 4-methylumbelliferyl-beta-D-glucopyranoside as substrate preincubated for 30 mins before sub...More data for this Ligand-Target Pair
Affinity DataIC50: 4.26nMpH: 7.0Assay Description:The assays were performed at 37°C with 4-MU-β-ᴅ-Glu as the substrate in Mcllvaine buffer (sodium citrate (100mM) and sodium phosphate...More data for this Ligand-Target Pair
Affinity DataIC50: 4.30nMAssay Description:Inhibition of lysosomal beta-glucosidase in human fibroblast using 4-methylumbelliferyl-beta-D-glucopyranoside as substrate preincubated for 24 hrs b...More data for this Ligand-Target Pair
Affinity DataIC50: 4.73nMpH: 7.0Assay Description:The assays were performed at 37°C with 4-MU-β-ᴅ-Glu as the substrate in Mcllvaine buffer (sodium citrate (100mM) and sodium phosphate...More data for this Ligand-Target Pair
Affinity DataIC50: 4.75nMpH: 7.0Assay Description:The assays were performed at 37°C with 4-MU-β-ᴅ-Glu as the substrate in Mcllvaine buffer (sodium citrate (100mM) and sodium phosphate...More data for this Ligand-Target Pair
Affinity DataIC50: 5nMpH: 7.0Assay Description:Competitive inhibition of recombinant Imiglucerase using 4-methylumbelliferyl-beta-D-glucopyranoside as substrate preincubated for 30 mins before sub...More data for this Ligand-Target Pair
Affinity DataIC50: 5nMpH: 7.0Assay Description:Competitive inhibition of recombinant Imiglucerase using 4-methylumbelliferyl-beta-D-glucopyranoside as substrate preincubated for 30 mins before sub...More data for this Ligand-Target Pair
Affinity DataIC50: 5.05nMpH: 7.0Assay Description:The assays were performed at 37°C with 4-MU-β-ᴅ-Glu as the substrate in Mcllvaine buffer (sodium citrate (100mM) and sodium phosphate...More data for this Ligand-Target Pair
Affinity DataIC50: 5.11nMpH: 7.0Assay Description:The assays were performed at 37°C with 4-MU-β-ᴅ-Glu as the substrate in Mcllvaine buffer (sodium citrate (100mM) and sodium phosphate...More data for this Ligand-Target Pair
Affinity DataIC50: 5.20nMAssay Description:The enzyme in GCase enzyme activity buffer (25 μL/well) was added to a 384-well black plate. The fluorescent probe 3 (25 nL/well, 50 nM final co...More data for this Ligand-Target Pair
Affinity DataIC50: 5.20nMAssay Description:The compounds in DMSO solution (0.5 μL/well) were transferred to a black 96-well plate (the final titration started from 100 μM, a 12 or 24...More data for this Ligand-Target Pair
Affinity DataIC50: 5.20nMpH: 5.9Assay Description:Inhibition of synthetic recombinant wild type GCase enzyme velaglucerase alfa (unknown origin) at pH 5.9 preincubated for 5 mins followed by addition...More data for this Ligand-Target Pair
Affinity DataIC50: 5.27nMpH: 5.2Assay Description:The assays were performed at 37°C with 4-MU-β-ᴅ-Glu as the substrate in Mcllvaine buffer (sodium citrate (100mM) and sodium phosphate...More data for this Ligand-Target Pair
Affinity DataIC50: 5.34nMpH: 5.2Assay Description:The assays were performed at 37°C with 4-MU-β-ᴅ-Glu as the substrate in Mcllvaine buffer (sodium citrate (100mM) and sodium phosphate...More data for this Ligand-Target Pair
Affinity DataIC50: 5.44nMpH: 5.2Assay Description:The assays were performed at 37°C with 4-MU-β-ᴅ-Glu as the substrate in Mcllvaine buffer (sodium citrate (100mM) and sodium phosphate...More data for this Ligand-Target Pair
Affinity DataIC50: 5.60nMAssay Description:The enzyme in GCase enzyme activity buffer (25 μL/well) was added to a 384-well black plate. The fluorescent probe 3 (25 nL/well, 50 nM final co...More data for this Ligand-Target Pair
Affinity DataKi: 3.06nM IC50: 5.80nMpH: 7.0Assay Description:The enzyme inhibition assays used monitored the ability of a test compound to bind and prevent the hydrolysis of a fluorogenic substrate in a concent...More data for this Ligand-Target Pair
Affinity DataIC50: 6nMAssay Description:The enzyme in GCase enzyme activity buffer (25 μL/well) was added to a 384-well black plate. The fluorescent probe 3 (25 nL/well, 50 nM final co...More data for this Ligand-Target Pair
Affinity DataIC50: 6nMAssay Description:The enzyme in GCase enzyme activity buffer (25 μL/well) was added to a 384-well black plate. The fluorescent probe 3 (25 nL/well, 50 nM final co...More data for this Ligand-Target Pair
Affinity DataIC50: 6.20nMAssay Description:Various concentrations of test compounds were prepared in DMSO and then diluted into buffer consisting of 50 mM sodium phosphate 0.25% w/v sodium tau...More data for this Ligand-Target Pair
Affinity DataIC50: 6.20nMpH: 7.0Assay Description:Various concentrations of test compounds were prepared in DMSO and then diluted into buffer consisting of 50 mM sodium phosphate 0.25% w/v sodium tau...More data for this Ligand-Target Pair
Affinity DataIC50: 6.23nMpH: 5.2Assay Description:The assays were performed at 37°C with 4-MU-β-ᴅ-Glu as the substrate in Mcllvaine buffer (sodium citrate (100mM) and sodium phosphate...More data for this Ligand-Target Pair
Affinity DataIC50: 6.30nMAssay Description:Various concentrations of test compounds were prepared in DMSO and then diluted into buffer consisting of 50 mM sodium phosphate 0.25% w/v sodium tau...More data for this Ligand-Target Pair
Affinity DataIC50: 6.30nMpH: 7.0Assay Description:Various concentrations of test compounds were prepared in DMSO and then diluted into buffer consisting of 50 mM sodium phosphate 0.25% w/v sodium tau...More data for this Ligand-Target Pair
Affinity DataIC50: 6.5nMAssay Description:The compounds in DMSO solution (0.5 μL/well) were transferred to a black 96-well plate (the final titration started from 100 μM, a 12 or 24...More data for this Ligand-Target Pair
In DepthDetails
Affinity DataIC50: 6.5nMAssay Description:The enzyme in GCase enzyme activity buffer (25 μL/well) was added to a 384-well black plate. The fluorescent probe 3 (25 nL/well, 50 nM final co...More data for this Ligand-Target Pair
Affinity DataIC50: 6.5nMAssay Description:Various concentrations of test compounds were prepared in DMSO and then diluted into buffer consisting of 50 mM sodium phosphate 0.25% w/v sodium tau...More data for this Ligand-Target Pair
Affinity DataIC50: 6.5nMpH: 5.9Assay Description:Inhibition of synthetic recombinant wild type GCase enzyme velaglucerase alfa (unknown origin) at pH 5.9 preincubated for 5 mins followed by addition...More data for this Ligand-Target Pair
Affinity DataIC50: 6.5nMpH: 7.0Assay Description:Various concentrations of test compounds were prepared in DMSO and then diluted into buffer consisting of 50 mM sodium phosphate 0.25% w/v sodium tau...More data for this Ligand-Target Pair
Affinity DataIC50: 6.60nMAssay Description:The compounds in DMSO solution (0.5 μL/well) were transferred to a black 96-well plate (the final titration started from 100 μM, a 12 or 24...More data for this Ligand-Target Pair
