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| Solution Information | help | |
| Enzyme: | cAMP-dependent protein kinase type II-beta regulatory subunit | |
| inhibitor: | BDBM81288 | |
| substrate: | n/a | |
| Solution Type: | Aqueous | |
| pH at Preparation: | n/a | |
| Temp. Prep.: | n/a | |
| Comments: | Assay Overview: The purpose of this biochemical assay is to determine dose response curves of compounds identified as able to induce the dissociation of the PKA-R2B complex in a set of previous experiments entitled, "Fluorescence polarization-based biochemical primary high throughput screening assay to identify activators of the Protein Kinase A-R2BA (PKA-R2B) complex" (AID 504700), that confirmed activity in a set of experiments entitled, "Fluorescence polarization-based biochemical high throughput confirmation assay for activators of the Protein Kinase A-R2B (PKA-R2B) complex" (AID 504797) and that were inactive in a set of experiments entitled, "Counterscreen for activators of the Protein Kinase A-R2B (PKA-R2B) complex: fluorescence polarization-based biochemical high throughput screening assay to identify fluorescence polarization assay artifacts" (AID 504796). In addition dose response curves were also determined for compounds showing activation of greater than 10% in a set of e | |
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