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Target
Serine/threonine-protein kinase Chk1
Ligand
BDBM14643
Substrate
Cdc25C peptide substrate
Meas. Tech.
Chk1 Enzymatic Assay
IC50
13±n/a nM
Citation
 Li, G; Hasvold, LA; Tao, ZF; Wang, GT; Gwaltney, SL; Patel, J; Kovar, P; Credo, RB; Chen, Z; Zhang, H; Park, C; Sham, HL; Sowin, T; Rosenberg, SH; Lin, NH Synthesis and biological evaluation of 1-(2,4,5-trisubstituted phenyl)-3-(5-cyanopyrazin-2-yl)ureas as potent Chk1 kinase inhibitors. Bioorg Med Chem Lett 16:2293-8 (2006) [PubMed]  Article
Target
Name:
Serine/threonine-protein kinase Chk1
Synonyms:
CHEK1 | CHK1 | CHK1 checkpoint homolog | CHK1_HUMAN | Checkpoint kinase-1 (CHK1)
Type:
Enzyme Catalytic Domain
Mol. Mass.:
54443.02
Organism:
Homo sapiens (Human)
Description:
gi_166295192
Residue:
476
Sequence:
MAVPFVEDWDLVQTLGEGAYGEVQLAVNRVTEEAVAVKIVDMKRAVDCPENIKKEICINKMLNHENVVKFYGHRREGNIQYLFLEYCSGGELFDRIEPDIGMPEPDAQRFFHQLMAGVVYLHGIGITHRDIKPENLLLDERDNLKISDFGLATVFRYNNRERLLNKMCGTLPYVAPELLKRREFHAEPVDVWSCGIVLTAMLAGELPWDQPSDSCQEYSDWKEKKTYLNPWKKIDSAPLALLHKILVENPSARITIPDIKKDRWYNKPLKKGAKRPRVTSGGVSESPSGFSKHIQSNLDFSPVNSASSEENVKYSSSQPEPRTGLSLWDTSPSYIDKLVQGISFSQPTCPDHMLLNSQLLGTPGSSQNPWQRLVKRMTRFFTKLDADKSYQCLKETCEKLGYQWKKSCMNQVTISTTDRRNNKLIFKVNLLEMDDKILVDFRLSKGDGLEFKRHFLKIKGKLIDIVSSQKIWLPAT
  
Inhibitor
Name:
BDBM14643
Synonyms:
3-(5-cyanopyrazin-2-yl)-1-[2-methoxy-4-(pyrrolidin-1-ylmethyl)phenyl]urea | N-pyrazinylurea Analog 29d
Type:
Small organic molecule
Emp. Form.:
C18H20N6O2
Mol. Mass.:
352.3904
SMILES:
COc1cc(CN2CCCC2)ccc1NC(=O)Nc1cnc(cn1)C#N
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
Cdc25C peptide substrate
Synonyms:
n/a
Type:
Peptide
Mol. Mass.:
2519.88
Organism:
n/a
Description:
Cdc25c peptide substrate contained a sequence of amino acid residues 204-225 of human phosphatase Cdc25C.
Residue:
22
Sequence:
AKVSRSGLYRSPSMPENLNRPR