| Assay Method Information | |
| | Protease Inhibition Assay |
| Description: | The inhibition assays were performed by mixing enzyme and fluorogenic substrate in the presence of inhibitor compounds. The hydrolysis of the substrate was recorded as an increase of the fluorescence intensity. Ki values were calculated from the IC50 values taken from a dose-response curve with the fluorescent assay using the equation Ki = (IC50 - [E]/2)/ (1 +[S]/Km), where [E] and [S] are the protease and substrate concentrations, Km is the Michaelis-Menten constant. |
| Affinity data for this assay | |
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