Assay Method Information

Assay Name:  Fluorescence Detection (FLIPR) Assay
Description:  The sample to be tested was dissolved in DMSO and diluted with detection buffer solution in 3-fold dilution. A reagent and a positive control were diluted in the same way. The reactions of the agonist, the reagent and the positive control were detected by a device of FLIPRTETRA with a total detection time of 180 s to estimate the ability to activate GPCR (S1P5) of each compound.
Affinity data for this assay
 

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