| Assay Method Information | |
| | Biochemical (FP) Assay |
| Description: | PARP1 and PARP2 protein were expressed, purified, and diluted in assay buffer containing 50 mM Tris pH 8.0, 0.001% Triton X-100, 10 mM MgCI2, 150 mM NaCI to a final concentration of 20 nM. The PARPi-FL was then added at a final concentration of 3 nM.The assay plate is centrifuged at 1000 rpm for 1 min and incubated for 4 h at room temperature.The fluorescent polarization is read using an Envision plate reader using the following settings: Excitation filter—FITC FP 480-Ex Slot 3, Emission filter-FITC FP P-pol 535-Em Slot 4, 2nd Emission filter—FITC FP S-pol 535-Em Slot 3, Mirror module-FITC FP Dual Enh-Slot 1. |
| Affinity data for this assay | |
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