| Assay Method Information | |
| | Receptor Binding Studies |
| Description: | Specific binding was determined by incubation of membranes from GHS-R1a transfected LLC cells with 125I-His9-ghrelin in the presence of increasing concentrations of compounds to obtain IC50. For the compounds behaving as agonists and displaying a high affinity binding for hGHS-R1a in radiolabeled binding experiments, the EC50 (the molar concentration of the agonist producing 50% of the maximal possible effect of that agonist) was determined using a dose-response curve. In the case of high-affinity antagonists, Kb values were determined using antagonist inhibition curves in the presence of 0.1 uM ghrelin (submaximal concentration). |
| Affinity data for this assay | |
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