| Assay Method Information | |
| | Enzyme Inhibition Assay |
| Description: | Enzyme reactions were carried out on Costar 96-well black assay plates. After the reaction, AMC fluorescence (excitation wavelength: 355 nm, emission wavelength: 460 nm) was subsequently measured 40 times every 3 s with a Wallac 1420 multi-label counter. Initial velocities (from 0 to 30 s) were used for IC50 determination, using GraphPad Prism 4 software. |
| Affinity data for this assay | |
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