| Assay Method Information | |
| | Luminescence Cell-Free Homogenous Dose Retest to Identify Inhibitors of Serine/Threonine Kinase 33 Activity |
| Description: | Keywords: STK33 Kinase, Non-ATP Competitive Inhibitor Assay Overview: Purified STK33 Kinase is preincubated with potential inhibitors and allowed to phosphorylate MBP at ~ 1.5 x Km ATP. Kinase activity is measured using the ADP Glo Kit (Promega) which converts ADP reaction product into a luminescent signal. Expected Outcome: Inhibitors of STK33 Kinase will cause a decreased luminescent readout. |
| Affinity data for this assay | |
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