| Assay Method Information | |
| | Inhibition Assay |
| Description: | The kinase-catalyzed phosphorylation of poly(Glu4-Tyr) in the presence of varying concentrations of inhibitor was determined. The kinase were employed as fusion proteins of glutathion-S-transferase (GST) and the kinase domain. The relative amount of phosphorylated substrate was quantified by antiphosphotyrosine enzyme-linked immunosorbent assay. |
| Affinity data for this assay | |
|---|---|
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