| Assay Method Information | |
| | Cyclooxygenase (COX) Inhibition Assay |
| Description: | The COX-1/COX-2 inhibition assay was done using an enzyme immunoassay (EIA) kit. The reaction buffer (960 uL), containing 0.1 M Tris-HCl (pH 8.0), 5 mM EDTA and 2 mM phenol, with either COX-1 or COX-2 (10 uL) enzyme in the presence of heme (10 uL) was added 10 uL of various concentrations of test drug solutions (0.01, 0.1, 1, 10, 50, and 100 uM in a final volume of 1 mL). The reaction was incubated for 5 min at 37 uC, followed by the addition of 10 uL of AA (100 uM) solution. The reaction was stopped by the addition of 50 uL of 1M HCl after 2 min. |
| Affinity data for this assay | |
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