| Assay Method Information | |
| | DPP-4 Activity Assay |
| Description: | The synthetic title compounds 7 (a-j) and 8 (a-j) were screened for in vitro DPP-4 inhibition using DPP-4 activity assay kit (Krishgen BioSystems). DPP-4 activity was determined by measuring the rate of hydrolysis of a surrogate substrate, H-Gly-Pro-7-amino-4-methylcoumarin (H-Gly-Pro-AMC) taking Litagliptin as standard. Screening of the compounds was done at 10 mM and 100 nM concentrations in triplicate. The compounds which showed good inhibition activity at 100 nM were further selected for IC50 values [Lai et al., J. Med. Chem., 45:560]. |
| Affinity data for this assay | |
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