| Assay Method Information | |
| | Biomol Green Assay |
| Description: | Biomol Green (Enzo) is a bioluminescent, homogeneous assay that measures ADP formed from a biochemical reaction. Because of its high sensitivity, the assay is suitable for monitoring enzyme activities at very early substrate conversions requiring very low amount of enzymes. This is critical since inhibitor potency has to be demonstrated at the cellular level where ATP is present at millimolar concentrations. The assay procedure used may be the same as in Zhang et al., Altered cofactor regulation with disease-associated p97/VCP mutations, Proc. Natl. Acad. Sci. USA, 112(14), E1705-E1714 (2015). |
| Affinity data for this assay | |
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