| Assay Method Information | |
| | MAOA and MAOB In Vitro Activity Assay |
| Description: | Principle: A specific luciferin derivative was used as a substrate. MAOA or MAOB can catalyze the conversion of substrate to luciferin methyl ester. The product luciferin methyl ester can produce fluorescent light under the action of luciferase, so that the activity of MAOA or MAOB can be reflected by the intensity of the fluorescent signal. Meanwhile, a blank control was set to determine the strength of the enzyme activity. Tranylcypromine was used as a positive inhibitor in the experiment.Sample treatment: The sample was dissolved in DMSO, and stored at low temperature. The concentration of DMSO in the final system was controlled to a range which would not affect the determined activity.In the initial screening, the activity of the sample was tested at a single concentration, for example 100 μM. For samples exhibiting activity under certain conditions, for example, the inhibition rate % Inhibition is greater than 50, the active dose-dependent relationship, i.e., the IC50 value was obtained by nonlinearly fitting the activity of the sample vs the concentration of the sample, and the software used for the calculation was Graphpad Prism 5. The model used for fitting was sigmoidal dose-response (varible slope), and for most inhibitor screening models, the bottom and top of the fitted curve were set to 0 and 100. |
| Affinity data for this assay | |
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