| Assay Method Information | |
| | COX Inhibitor Screening Assay |
| Description: | The COX enzyme activities were measured using the COX Inhibitor Screening Assay kit provided by Cayman (Cayman, Chemical Co., Ann Arbor, MI). The assay directly measures PGF2a produced by SnCl2 reduction of COX-derived PGH2. The prostanoid production was quantified via enzyme immunoassay using a broadly specific antibody that binds to all the major prostaglandin compounds. IC50 values were calculated for the most active compounds. Naproxen and indomethacin were used as the positive controls. |
| Affinity data for this assay | |
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