| Assay Method Information | |
| | Reporter Gene Assay |
| Description: | Human embryonic kidney (HEK) 293 cells were cultured in D-MEM medium. Transfections were performed by the calcium phosphate coprecipitation method. Test compounds with or without 100 nM T0901317 were added 8 h after the transfection. After overnight incubation, luciferase and beta-galactosidase activities were assayed using a luminometer. |
| Affinity data for this assay | |
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