Assay Method Information | |
| ChEMBL_1913972 (CHEMBL4416555) |
Description: | Competitive inhibition of N-terminal GST-tagged recombinant human PTP1B catalytic domain (1 to 321 residues) expressed in Escherichia coli assessed as inhibitory constant using pNPP as substrate preincubated for 10 mins followed by incubation with substrate for 30 mins and measured every 5 mins for 30 mins by Lineweaver-burk plot based Dixon plot analysis |
Affinity data for this assay | |
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